NFAT-Luc/HEK293
CBP74028
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| I. Background | |
| The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. | |
| II. Description | |
| This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | NFAT-Luciferase |
| Stability: | 32 passages |
| Synonym(s): | Nuclear factor of activated T cells; T cell |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10% FBS+300 ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. NFAT Luciferase Reporter - HEK293 Recombinant Cell Line (C8). |
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Figure 2. NFAT Luciferase Reporter -HEK293 Recombinant Cell Line (C4). |
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