STAT3-Luc/HEK293
CBPB0002
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| I. Background | |
| 癌症相关炎症的特征是存在特定的炎症细胞和炎症介质，包括细胞因子和趋化因子。 最近的证据表明，信号转导和转录激活因子（STAT）家族蛋白，特别是 STAT3，在恶性转 化开始和癌症进展过程中，在选择性诱导和维持癌前炎症微环境中起着至关重要的作用。 信号转导和转录激活因子 3 (STAT3)是一种转录激活因子，它将细胞外受体的信号直 接传递到细胞核，介导一些基因的表达，在细胞凋亡等细胞过程中发挥重要作用。在细胞 因子和生长因子的作用下，STAT3 被受体相关 Janus 激酶(JAK) 磷酸化，形成同二聚体或异 二聚体，并转位到细胞核中，作为转录激活剂发挥作用。STAT3 信号传导是恶性肿瘤的主 要内在途径，因为它在恶性细胞中频繁激活，并且在调节肿瘤微环境中对癌症发炎至关重 要的许多基因中起关键作用。 |
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| II. Description | |
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STAT3-Luc HEK293 报告基因细胞，是由 STAT3 调控并表达 Luc 荧光素 酶报告基因的HEK293细胞。IL6细胞因子与受体复合物结合，启动JAK/STAT3信号通路， 原理见下图所示。
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | STAT3-Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10%FBS+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
Ⅴ. Representative Data |
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Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Induction of STAT3 activity by hIL6 in STAT3-Luc HEK293 Cells (C7).  |
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Figure 3. Inhibition of IL-6-induced Reporter Activity by IL-6 Neutralizing Antibody in STAT3-Luc HEK293 Cells. |
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